KMID : 1134820080370081044
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Journal of the Korean Society of Food Science and Nutrition 2008 Volume.37 No. 8 p.1044 ~ p.1048
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The Stabilization of Lactic Acid Bacteria, Bacillus polyfermenticus KJS-2
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Kim Kang-Min
Hong Yong-Geun Lee Sang-Kil Lee Jin-Young Kang Jae-Seon
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Abstract
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Bacillus polyfermenticus KJS-2 (Bp2) was isolated from Bispan¨Þ, a commercially available probiotics
consisting of more than 4 strains. The objective of this study was to investigate the effect of three-layer
coating on the stabilty of Bp2. Bp2 was microencapsulated with sodium alginate using an air atomizer. The
Bp2 loaded pellets were also coated with HFP-chitosan and HPMCP for oral delivery system. When compared
to the uncoated Bp2, the survival of the three-layer coated Bp2 increased to approximately 63% (p<0.01) in
a 30% ethanol solution, 54% (p<0.05) in an artificial gastric juice (pH 2), and 53% (p<0.05) in the bile acid
(pH 5). When coated beads were stored at 100oC and 130oC, Bp2 in coated beads was very stable (p<0.01)
compared to uncoated Bp2.
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KEYWORD
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Bacillus polyfermenticus KJS-2, three-layer coating, alginate, stability
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